Anti-Coronin 1a antibody (ab14787) at 0.3 µg/ml + Cell lysates prepared from Jurkat cells at 35 µg
ab14787 staining Coronin 1a in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab14787 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated rabbit anti-goat polyclonal, used at a 1/50 dilution. Nuclei are counterstained with DAPI.See Abreview
ab14787 staining Coronin 1a in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab14787 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated rabbit anti-goat polyclonal, used at a 1/50 dilution. Nuclei are counterstained with DAPI.See Abreview