Anti-Corticotropin Releasing Factor antibody

• Supplier: Abcam
• Catalog: ab8901
• Prices: $398.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF RIA WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to amino acids 1-41 of Rat Corticotropin Releasing Factor
• Description: Rabbit Polyclonal
• Gene: CRH
• Conjugate: Unconjugated

Product images

Anti-Corticotropin Releasing Factor antibody (ab8901) at 1/2500 dilution + Mouse hypothalamus whole tissue lysate at 50 µgSecondaryHRP-conjugated Donkey anti-rabbit IgG polyclonal - undiluteddeveloped using the ECL techniquePerformed under non-reducing conditions.Observed band size : 25 kDa (why is the actual band size different from the predicted?)Additional bands at : 17 kDa (possible non-specific binding),23 kDa (possible non-specific binding),35 kDa (possible non-specific binding).Exposure time : 1 minuteThis image is courtesy of an Abreview submitted by Jean-Christophe HelblingSee Abreview

Radioimmunoassay using ab8901 - Day 1: Add Recombinant Protein A/G (10ug/ml) to a 96 wells plate; parafilm wrap; incubate overnight at 4°C. - Day 2: Aspirate and wash the plate to remove loosely bound Protein A/G; add antibody solution (dilution 1:50000); parafilm wrap; incubate 24h at 4°C. - Day 3: Aspirate and wash the plate to remove loosely bound antibody; add CRF standard (0, 0.03, 0.1, 0.3, 1, 3, 10, 30 ng/ml); parafilm wrap; incubate 24h at 4°C. - Day 4: DO NOT ASPIRATE; add 125I-CRF; incubate 24h at 4°C. - Day 5: Aspirate and wash the plate; break the plate and collect the wells in scintillation tubes; add 2ml of scintillation liquid; count in beta counter (open the 3H window to at least 30-40 keV), at least 2min/tube.

Immunohistochemical analysis of rat brain tissue, staining Corticotropin Releasing Factor with ab8901. Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. The figure on the left is a secondary only control.See Abreview

Product References

Administration of antenatal glucocorticoids and postnatal surfactant ameliorates - Administration of antenatal glucocorticoids and postnatal surfactant ameliorates

Cuevas Guaman M, Sbrana E, Shope C, Showalter L, Hu M, Meloche S, Aagaard K. Pediatr Res. 2014 Jul;76(1):24-32.

Epigenetic modulation of chronic anxiety and pain by histone deacetylation. - Epigenetic modulation of chronic anxiety and pain by histone deacetylation.

Tran L, Schulkin J, Ligon CO, Greenwood-Van Meerveld B. Mol Psychiatry. 2014 Oct 7.

A neuronal migratory pathway crossing from diencephalon to telencephalon - A neuronal migratory pathway crossing from diencephalon to telencephalon

Garcia-Moreno F, Pedraza M, Di Giovannantonio LG, Di Salvio M, Lopez-Mascaraque L, Simeone A, De Carlos JA. Nat Neurosci. 2010 Jun;13(6):680-9.

Immunohistochemical localization and functional characterization of somatostatin - Immunohistochemical localization and functional characterization of somatostatin

Ruggeri RM, Ferrau F, Campenni A, Simone A, Barresi V, Giuffre G, Tuccari G, Baldari S, Trimarchi F. Eur J Histochem. 2009 Jan-Mar;53(1):1-6.

Loss of glucocorticoid receptor function in the pituitary results in early - Loss of glucocorticoid receptor function in the pituitary results in early

Erdmann G, Schutz G, Berger S. Endocrinology. 2008 Jul;149(7):3446-51.

Loss of the limbic mineralocorticoid receptor impairs behavioral plasticity. - Loss of the limbic mineralocorticoid receptor impairs behavioral plasticity.

Berger S, Wolfer DP, Selbach O, Alter H, Erdmann G, Reichardt HM, Chepkova AN, Welzl H, Haas HL, Lipp HP, Schutz G. Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):195-200. Epub 2005 Dec 20.