Anti-COUP TF1 antibody [EPR10841]

• Supplier: Abcam
• Catalog: ab181137
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10841
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide within Human COUP TF1 aa 1-100
• Description: Rabbit Monoclonal
• Gene: NR2F1
• Conjugate: Unconjugated

Product images

All lanes : Anti-COUP TF1 antibody [EPR10841] (ab181137) at 1/1000 dilutionLane 1 : Human fetal brain lysate with NFDM/TBSTLane 2 : Human fetal kidney lysate with NFDM/TBSTLane 3 : 293 cell lysate with NFDM/TBSTLysates/proteins at 20 µg per lane.Blocking peptides at 5 % per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Immunohistochemical analysis of paraffin-embedded Human glioblastoma staining COUP TF1 using ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma staining COUP TF1 using ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.  

Immunofluorescence analysis of COUP TF1 in 4% paraformaldehyde fixed 293 cells using ab181137 at 1/100 (red). Alexa Fluor 555 conjugated Goat anti Rabbit IgG was used as a secondary antibody at 1/200 dilution, with Dapi staining (blue).

Flow cytometric analysis of COUP TF1 labeling 2% paraformaldehyde fixed 293 cells using ab181137 at 1/70 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.