ab66739, at 2 µg/ml, staining COX IV in human kidney tissue by immunohistochemistry on paraffin-embedded tissue. Positively stained cells are epithelial cells of the renal tubule.
ab66739, at 2 µg/ml, staining COX IV in Human heart tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained myocardial cells.
ICC/IF image of ab66739 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66739 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-COX IV antibody (ab66739) at 1.25 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
All lanes : Anti-COX IV antibody (ab66739)Lane 1 : Lysate prepared from human liverLane 2 : Lysate prepared from rat liverLane 3 : Lysate prepared from wild type mouse liverLane 4 : Lysate prepared from AMPKa1+2-/- mouse liverLane 5 : Lysate prepared from human muscleLane 6 : Lysate prepared from rat muscleLane 7 : Lysate prepared from mouse muscle
