Immunohistochemical analysis of paraffin-embedded Human colorectal carcinoma with COX IV Mouse mAb [4D11-B3-E8] (1:50),showing cytoplasm localization. A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
All lanes : Anti-COX IV antibody [4D11-B3-E8] (ab131177) at 1/5000 dilutionLane 1 : COS7 cell lysateLane 2 : 3T3 cell lysateLane 3 : K562 cell lysateLane 4 : C6 cell lysateLane 5 : Goat muscle cell lysateLane 6 : CHO-K1 cell lysateLane 7 : HeLa cell lysate
Immunofluorescent analysis of HeLa cells labelling COXIV Isoform 2 with ab131177 at 1/150 dilution.
Immunoprecipitation analysis of HeLa cell lysate labeling COX IV with ab131177.
All lanes : Anti-COX IV antibody [4D11-B3-E8] (ab131177) at 1/1000 dilutionLane 1 : Mouse skeletal muscle lysateLane 2 : Jurkat cell lysate
Flow Cytometric analysis of K562 cells labelling COXIV Isoform 2 with ab131177 (red) at 1/100 dilution; followed by FITC-conjugated goat anti-mouse IgG. Mouse IgG control (blue).