All lanes : Anti-COX17 antibody (ab69611) at 1/500 dilutionLane 1 : Extracts from HeLa cellsLane 2 : Extracts from HepG2 cellsLane 3 : Extracts from COLO cellsLane 4 : Extracts from 293 cellsLane 5 : Extracts from COLO cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
ab69611, at 1/50 dilution, staining human liver carcinoma tissue in the presence or absence of the immunising peptide.
ICC/IF image of ab69611 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69611, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.