Anti-COX4NB antibody (ab168785) at 1/500 dilution + Human placenta tissue lysate at 50 µg
All lanes : Anti-COX4NB antibody (ab168785) at 1/500 dilutionLane 1 : COX4NB transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 15 µl per lane.
Immunofluorescence analysis of paraformaldehyde-fixed HepG2 cells, labeling COX4NB using ab168785 at 20 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining. ab168785 should be Protein A purified before use.