All lanes : Anti-COX5A antibody [EPR14208(B)] (ab180129) at 1/5000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : A431 cell lysateLane 4 : Human fetal heart lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of HeLa cells labeling COX5A using ab180129 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5A using ab180129 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.
Immunofluorescence analysis of HeLa cells labeling COX5A using ab180129 at 1/100 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Couterstain: DAPI.
Flow cytometry analysis of HeLa cells labeling COX5A using ab180129 at 1/200 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.