Anti-COX5B antibody (ab88440) at 1 µg/ml + human pancreas tissue lysate at 50 µgdeveloped using the ECL technique
Anti-COX5B antibody (ab88440) at 1 µg/ml + HepG2 cell lysate at 50 µgdeveloped using the ECL technique
All lanes : Anti-COX5B antibody (ab88440) at 1 µg/mlLane 1 : cell lysate from COX5B transfected 293T cellsLane 2 : cell lysate from non transfected 293T cellsLysates/proteins at 25 µg per lane.developed using the ECL technique
ab88440 at 10 µg/ml staining COX5B in paraformaldehyde fixed, permeabilised HeLa cells by immunofluorescence.
IHC image of COX5B staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88440, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.