Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/50000 dilution + MCF7 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/10000 dilution + Human skin cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.
Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 with ab181255 at a 1/250 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) (green) was used at a 1/200 dilution. DAPI staining in blue.
Flow cytometry analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 (red) with ab181255 at a 1/80 dilution, or negative control rabbit monoclonal IgG (green). As secondary antibody goat anti rabbit IgG (FITC) was used at a 1/150 dilution.