Anti-CRCP antibody [EPR9670(B)]

• Supplier: Abcam
• Catalog: ab139264
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9670(B)
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CRCP aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: CRCP
• Conjugate: Unconjugated

Product images

All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/5000 dilution (unpurified)Lane 1 : Jurkat cell lysateLane 2 : Mouse kidney tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/10000 dilution (purified)Lane 1 : Jurkat cell lysateLane 2 : Mouse kidney tissue lysateLane 3 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-CRCP antibody [EPR9670(B)] (ab139264) at 1/1000 dilution (unpurified)Lane 1 : Human ovary cancer lysateLane 2 : HeLa lysateLane 3 : Jurkat lysateLane 4 : Human prostate cancer lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with purified ab139264 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling CRCP with unpurified ab139264 at 1/50.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CRCP with unpurified ab139264 at 1/50.

Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with unpurified ab139264 at 1/100. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunocytochemsitry/Immunofluorescence analysis of LnCap cells labelling CRCP (red) with purified ab139264 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CRCP with unpurified ab139264 at 1/100.

ab139264 (unpurified) at 1/50 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

ab139264 (purified) at 1/100 immunoprecipitating CRCP in human prostate cancer tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.