Anti-CREB (phospho S133) antibody [E113]
| Name | Anti-CREB (phospho S133) antibody [E113] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32096 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E113 |
| Applications | WB IHC-P FC IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Chicken, Bovine, Zebrafish |
| Antigen | Synthetic phoshopeptide corresponding to residues surrounding Serine 133 of human CREB |
| Blocking Peptide | CREB (phospho S133) peptide |
| Description | Rabbit Monoclonal |
| Gene | CREB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing A431 cells stained with ab32096 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32096, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-CREB (phospho S133) antibody [E113] (ab32096) at 1/500 dilutionLane 1 : A431 cells untreated.Lane 2 : A431 cells treated with forskolin.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/2095_ab32089_1.jpg)
All lanes : Anti-CREB (phospho S133) antibody [E113] (ab32096) at 1/500 dilutionLane 1 : A431 cells untreated.Lane 2 : A431 cells treated with forskolin.
Ab32096, at a 1/250 dilution, staining CREB in A431 cells by Immunofluorescence. Image A: Cells are untreated. Image B: Cells are treated with Phosphatase.
Ab32096, at a 1/250 dilution, staining CREB in paraffin embedded human thyroid gland adenocarcinoma tissue by Immunohistochemistry. Image A: Cells are untreated. Image B: Cells are treated with Phosphatase.
ab32096 staining CREB in Rat hippocampus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Sections were formalin-fixed, and subjected to antigen retrieval by autoclave prior to blocking with 8% milk for 30 minutes at 37°C. The primary antibody was diluted 1/100 with DAKO antibody diluent and incubated with the sample for 18 hours at 4°C. An LSAB-labeled Streptavidin-Biotin conjugated Goat polyclonal antibody was used undiluted as the secondary antibody.See Abreview
developed using the ECL techniquePerformed under reducing conditions.
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