Anti-CREG1 [EPR13279] antibody

• Supplier: Abcam
• Catalog: ab191562
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR13279
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CREG1 aa 1-100
• Description: Rabbit Monoclonal
• Gene: CREG1
• Conjugate: Unconjugated

Product images

All lanes : Anti-CREG1 [EPR13279] antibody (ab191562) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : 293 cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded, Human cervix carcinoma tissue labeling CREG1 with ab191562 at a 1/100 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CREG1 with ab191562 at a 1/50 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PRMT3 at a 1/50 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling CREG1 using ab191562 at 1/40 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.