Anti-CREG1 antibody (ab68341) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Anti-CREG1 antibody (ab68341) at 5 µg/ml + HL60 cell lysate at 25 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
ab68341 staining CREG1 on formalin-fixed paraffin-embedded human adrenal gland tissue. Antibody concentration 3 ug/ml. Antigen retrieval in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes was performed.
Detection limit for recombinant GST tagged CREG1 is approximately 0.3ng/ml as a capture antibody.