Anti-CREG1 antibody [EPR13735] (ab181844) at 1/1000 dilution + Human fetal lung lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunofluorescence analysis of K562 cells labeling CREG1 using ab181844 at 1/100 dilution. A goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with acetone at -20°C. Counterstain: DAPI.
Anti-CREG1 antibody [EPR13735] (ab181844) at 1/1000 dilution + Human fetal heart lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of Human fetal heart lysate labeling CREG1 using ab181844 at 1/60 dilution (Lane 1). An anti-rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: negative control.
Immunofluorescence analysis of 293T cells labeling CREG1 using ab181844 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.