All lanes : Anti-CRMP3 antibody - ChIP Grade (ab36217)Lane 1 : E.coli transformed with cDNA of human CRMP1Lane 2 : E.coli transformed with cDNA of human CRMP2Lane 3 : E.coli transformed with cDNA of human CRMP3Lane 4 : E.coli transformed with cDNA of human CRMP4Lane 5 : E.coli transformed with cDNA of human CRMP5
IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.