Anti-CRNKL1 antibody [EPR16064] (ab192248) at 1/20000 dilution + Human seminoma lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling CRNKL1 with ab192248 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are ab192248 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.