All lanes : Anti-CRSP9 antibody [EPR15410] (ab187146) at 1/1000 dilutionLane 1 : Jurkat lysateLane 2 : 293T lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-CRSP9 antibody [EPR15410] (ab187146) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometrical analysis of HeLa cells labeling CRSP9 with ab187146 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
Immunohistochemical analysis of paraffin embedded Mouse brain tissue labeling CRSP9 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.
Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling CRSP9 with ab187146 at 1/100 with prediluted (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.