All lanes : Anti-CRTAP antibody [EPR15122(B)] (ab183720) at 1/20000 dilutionLane 1 : Saos-2 whole cell lysateLane 2 : SW1353 whole cell lysateLane 3 : HeLa whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-CRTAP antibody [EPR15122(B)] (ab183720) at 1/5000 dilution + A431 whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human trachea tissue sections labeling CRTAP using ab183720 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue sections labeling CRTAP using ab183720 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Lysate from SW1353 cells was immunoprecipitated with ab183720 at a 1/50 dilution. For the subsequent blot, ab183720 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Lane 2: TBS instead of SW1353 lysates. Blocking/ Dilution buffer: 5% NFDM/TBST.