Anti-CSDE1 antibody [EPR17414]

• Supplier: Abcam
• Catalog: ab201688
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17414
• Applications: ICC/IF ICC/IF IP WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CSDE1 aa 400-500
• Description: Rabbit Monoclonal
• Gene: CSDE1
• Conjugate: Unconjugated

Product images

All lanes : Anti-CSDE1 antibody [EPR17414] (ab201688) at 1/10000 dilutionLane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) lysateLane 2 : HEK293 (Human embryonic kidney)Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-CSDE1 antibody [EPR17414] (ab201688) at 1/1000 dilution + Human testis at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-CSDE1 antibody [EPR17414] (ab201688) at 1/1000 dilutionLane 1 : Mouse brainLane 2 : Rat brainLane 3 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CSDE1 with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human breast carcinoma tissue is observed.Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CSDE1 with ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.Cytoplasmic staining on Human kidney tissue is observed.Counter stained with Hematoxylin.Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CSDE1 with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line is observed.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows;1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CSDE1 with ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing cytoplasmic staining on MCF7 cell line is observed.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows;1. ab201688 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

CSDE1 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab201688 at 1/40 dilution.Western blot was performed from the immunoprecipitate using ab201688 at 1/1500 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as secondary antibody at 1/1500 dilution.Lane 1: K562 whole cell extract 10 µg (Input).Lane 2: ab201688 IP in K562 whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab20688 in K562 whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 second