Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61770 at a dilution of 1/50. left: untreated sample. Right: sample treated with immunising peptide.
All lanes : Anti-CSEN antibody (ab61770) at 1/500 dilutionLane 1 : extracts from A431 cells with no peptideLane 2 : extracts from A431 cells with peptide
ICC/IF image of ab61770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61770, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.