Anti-CSN1 antibody (ab169313) at 1/500 dilution + Human spleen lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
All lanes : Anti-CSN1 antibody (ab169313) at 1/500 dilutionLane 1 : CSN1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of permeabilized HeLa cells labeling CSN1 with purified ab169313 at 10µg/ml. Antibody purification with Protein A will be needed prior to use.