All lanes : Anti-CtBP1 (phospho S422) antibody (ab92577) at 1/500 dilutionLane 1 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)Lane 2 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)Lane 3 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins) with immunizing phosphopeptide at 10 µgLysates/proteins at 30 µg per lane.
Left panel: ab92577, at a 1/50 dilution, staining phosphorylated CtBP1 in formalin/ fixed, paraffin embedded Human tissue brain, using Immunohistochemistry. Right panel: same, after pre-incubation with the immunizing phosphopeptide.