Anti-CTHRC1 antibody - N-terminal (ab135716) at 1/100 dilution + A375 cell line lysates at 35 µg
All lanes : Anti-CTHRC1 antibody - N-terminal (ab135716) at 1/100 dilutionLane 1 : 293 cell lysates non transfectedLane 2 : 293 cell lysates transiently transfected with the CTHRC1 geneLysates/proteins at 2 µg per lane.
Immunohistochemical analysis of CTHRC1 expression in paraffin embedded, formalin fixed Human skin tissue labelled with ab135716 at 1/50 dilution. This was followed by incubation with a peroxidase conjugated secondary antibody and DAB staining.
Flow cytometric analysis of CTHRC1 expression in A375 cells labelled with ab135716 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.