All lanes : Anti-CTRP1 antibody (ab83177) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate Lane 5 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.