cyclin H (C-18): sc-609. Western blot analysis of cyclin H expression in cell extracts (UPPER PANEL) and purified polyhistidine tagged cyclin H fusion protein (LOWER PANEL). Cells tested include Jurkat (A), A-431 (B) and K-562 (C). Antibody concentrations tested for analysis of recombinant cyclin H protein include 0.1 µg/ml (D), 0.05 µg/ml (E), 0.01 µg/ml (F) and 0.001 µg/ml (G).
Western blot analysis of cyclin H expression in Jurkat (A), A-431 (B), K-562 (C), C32 (D,E) and NIH/3T3 (F) whole cell lysates. Antibodies tested include cyclin H (C-18): sc-609 (A-D) and cyclin H (FL-323): sc-855 (E,F).
cyclin H (C-18): sc-609. Western blot analysis of cyclin H expression in non-transfected 293: sc-110760 (A), human cyclin H transfected: sc-159947 (B) 293 and A-673 (C) whole cell lysates.