Anti-CXCL11 antibody [10C6]

• Supplier: Abcam
• Catalog: ab155936
• Prices: $376.00
• Sizes: 200 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 10C6
• Applications: WB ELISA
• Species Reactivities: Human
• Antigen: Recombinant full length Human CXCL11 expressed in E
• Description: Mouse Monoclonal
• Gene: CXCL11
• Conjugate: Unconjugated

Product images

All lanes : Anti-CXCL11 antibody [10C6] (ab155936) at 1/1000 dilutionLane 1 : Recombinant Human CXCL11Lane 2 : Induced HeLa cell culture supernatantLysates/proteins at 0.25 µg per lane.SecondaryGoat anti-mouse HRP conjugated antibody at 1/10000 dilutiondeveloped using the ECL technique

Sandwich ELISA analysis of Human CXCL11 was performed using an ELISA Kit by coating a blank 96-well microtiter plate with 100µl per well of ab155936 in duplicate at 1, 3, 4, 5, 7, and 9µg/ml in DPBS and incubating for 12-18 hours at 4C. The plate was aspirated and blocked with 300µl per well of 4% BSA and 5% sucrose in DPBS for 1 hour at room temperature. Human CXCL11 recombinant protein at 50µl per well was added in duplicate at 4000, 1600, 640, 256, 102.4, 40.96, and 0 pg/ml for 2 hours at room temperature along with 50µl of a Human CXCL11 biotinylated monoclonal antibody in all applicable wells at 0.2µg/ml for 2 hours at room temperature. The plate was washed and incubated with 100µl per well of Streptavidin-HRP in all test wells at 1:80,000 dilution for 1 hour at room temperature and then washed and incubated with 100µl per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotomet