Anti-CXCL15 antibody [EPR14470]

• Supplier: Abcam
• Catalog: ab197016
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR14470
• Applications: IHC-P IP WB
• Species Reactivities: Mouse
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse CXCL15 aa 100 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: Cxcl15
• Conjugate: Unconjugated

Product images

All lanes : Anti-CXCL15 antibody [EPR14470] (ab197016) at 1/5000 dilutionLane 1 : Mouse lung tissue lysateLane 2 : Mouse brain tissue lysateLane 3 : Mouse heart tissue lysateLane 4 : Mouse kidney tissue lysateLane 5 : Mouse spleen tissue lysateLane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

CXCL15 was immunoprecipitated from Mouse lung lysates with ab197016 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197016 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Mouse lung lysate 10 µg (Input). Lane 2: ab197016 IP in Mouse lung lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197016 in Mouse lung lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.