Anti-CXCR4 antibody [UMB2]

• Supplier: Abcam
• Catalog: ab124824
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: UMB2
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human, Rat
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CXCR4 aa 300 to the C-terminus (C terminal)
• Blocking Peptide: CXCR4 peptide
• Description: Rabbit Monoclonal
• Gene: CXCR4
• Conjugate: Unconjugated

Product images

Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab124824 at a working dilution of 1/500. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Anti-CXCR4 antibody [UMB2] (ab124824) at 1/100 dilution (purified) + Rat muscle tissue lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-CXCR4 antibody [UMB2] (ab124824) at 1/100 dilution (purified) + NIH/3T3 cell lysate at 20 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Anti-CXCR4 antibody [UMB2] (ab124824) at 1/100 dilution (purified) + WI-38 cell lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunofluorescence staining of Jurkat cells with purified ab124824 at a working dilution of 1 in 250, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab124824 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

Unpurified ab124824, at 1/50 dilution, staining CXCR4 in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.

Unpurified ab124824, at 1/50 dilution, staining CXCR4 in paraffin-embedded Human ovarian adenocarcinoma tissue by immunohistochemistry.

Unpurified ab124824, at 1/50 dilution, staining CXCR4 in paraffin-embedded Human tonsil tissue by immunohistochemistry.

Lane 1 : Anti-CXCR4 antibody [UMB2] (ab124824) at 1/500 dilution (unpurified)Lane 2 : Anti-CXCR4 antibody [UMB2] (ab124824) at 1/500 dilutionLane 1 : HEK239 tansfected with a CXCR4 (mouse) expression vector cell lysateLane 2 : HEK239 tansfected with an empty expression vector cell lysateLysates/proteins at 100000 cells per lane.SecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Product References

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CXCR4 prevents dispersion of granule neuron precursors in the adult dentate - CXCR4 prevents dispersion of granule neuron precursors in the adult dentate

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Hierarchical organization of multi-site phosphorylation at the CXCR4 C terminus. - Hierarchical organization of multi-site phosphorylation at the CXCR4 C terminus.

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