All lanes : Anti-CXCR5 antibody (ab46218) at 1 µg/mlLane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 3 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 4 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3951)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
IHC image of ab46218 staining CXCR5 in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) second test for 20 mins. The section was then incubated with ab46218, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.