Anti-Cyclin A2 antibody
| Name | Anti-Cyclin A2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7956 |
| Prices | $385.00 |
| Sizes | 1 ml |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F IP WB IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: HSKYHSVSLLNPPETLSV , corresponding to C terminal amino acids 405-422 of Mouse Cyclin A |
| Blocking Peptide | Cyclin A2 peptide |
| Description | Rabbit Polyclonal |
| Gene | CCNA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab7956 at 1/150 staining developing mouse embryo tissue sections (5 microns) by IHC-P. The tissue was formaldehyde fixed and and heat mediated antigen retrieval step in citric acid buffer was performed. The tissue was blocked in BSA and incubated with the antibody for 2 hours. A biotinylated goat antibody was used as the secondary. The image shows T/S of developing oesophagus, trachea, skeletal muscle and blood vessel wall.See Abreview
ICC/IF image of ab7956 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7956, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Li Y, Hu S, Ma G, Yao Y, Yan G, Chen J, Li Y, Zhang Z. Am J Transl Res. 2013 Apr 19;5(3):327-35. Print 2013.
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Li YG, Ji DF, Zhong S, Liu PG, Lv ZQ, Zhu JX, Chen JE, Chen HP. J Ethnopharmacol. 2013 Oct 28;150(1):187-95.
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Zhong S, Ji DF, Li YG, Lin TB, Lv ZQ, Chen HP. Chem Biol Interact. 2013 Nov 25;206(2):222-9.
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Sivilia S, Lorenzini L, Giuliani A, Gusciglio M, Fernandez M, Baldassarro VA, Mangano C, Ferraro L, Pietrini V, Baroc MF, Viscomi AR, Ottonello S, Villetti G, Imbimbo BP, Calza L, Giardino L. BMC Neurosci. 2013 Apr 5;14:44.
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Li L, Cheung T, Chen J, Herrup K. Int J Alzheimers Dis. 2011;2011:171464.
Discovery of a distinct domain in cyclin A sufficient for centrosomal - Discovery of a distinct domain in cyclin A sufficient for centrosomal
Pascreau G, Eckerdt F, Churchill ME, Maller JL. Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):2932-7. doi:
Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial - Lack of CCAAT enhancer binding protein beta (C/EBPbeta) in uterine epithelial
Ramathal C, Bagchi IC, Bagchi MK. Mol Cell Biol. 2010 Apr;30(7):1607-19.
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Tizon B, Ribe EM, Mi W, Troy CM, Levy E. J Alzheimers Dis. 2010;19(3):885-94.
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Sebastian S, Sreenivas P, Sambasivan R, Cheedipudi S, Kandalla P, Pavlath GK, Dhawan J. Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4719-24. doi:
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Roa W, Zhang X, Guo L, Shaw A, Hu X, Xiong Y, Gulavita S, Patel S, Sun X, Chen J, Moore R, Xing JZ. Nanotechnology. 2009 Sep 16;20(37):375101.