Anti-Cyclin B2 antibody [X29.2]
| Name | Anti-Cyclin B2 antibody [X29.2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab18250 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | X29.2 |
| Applications | IHC-P ICC/IF ICC/IF WB FC |
| Species Reactivities | Mouse, Rat, Human, Xenopus |
| Antigen | Full length protein (Xenopus laevis) |
| Description | Mouse Monoclonal |
| Gene | CCNB2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Cyclin B2 antibody [X29.2] (ab18250) at 2 µg/mlLane 1 : Whole cell lysate of Mitotic Hela cells (arrested by the spindle checkpoint due to depolymerization of microtubles by exposure to Nocodazole)Lane 2 : Whole cell lysate of Interphase Hela Cells, forced to exit mitosis by exposure to a Cdk1 inhibitor (exit from mitosis results in the degradation of Cyclin B2)SecondaryHRP-Conjugated Goat anti-Mouse diluted 1/20000developed using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3903_ab18250_1a.jpg)
All lanes : Anti-Cyclin B2 antibody [X29.2] (ab18250) at 2 µg/mlLane 1 : Whole cell lysate of Mitotic Hela cells (arrested by the spindle checkpoint due to depolymerization of microtubles by exposure to Nocodazole)Lane 2 : Whole cell lysate of Interphase Hela Cells, forced to exit mitosis by exposure to a Cdk1 inhibitor (exit from mitosis results in the degradation of Cyclin B2)SecondaryHRP-Conjugated Goat anti-Mouse diluted 1/20000developed using the ECL technique
IHC image of ab18250 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18250, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing HeLA cells stained with ab18250 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18250, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/3905_Cyclin-B2-Primary-antibodies-ab18250-3.jpg)
Overlay histogram showing HeLA cells stained with ab18250 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18250, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in - Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in
Dupre A, Daldello EM, Nairn AC, Jessus C, Haccard O. Nat Commun. 2014;5:3318.
Development of a conditionally immortalized human pancreatic beta cell line. - Development of a conditionally immortalized human pancreatic beta cell line.
Scharfmann R, Pechberty S, Hazhouz Y, von Bulow M, Bricout-Neveu E, Grenier-Godard M, Guez F, Rachdi L, Lohmann M, Czernichow P, Ravassard P. J Clin Invest. 2014 May;124(5):2087-98.
When the genome plays dice: circumvention of the spindle assembly checkpoint and - When the genome plays dice: circumvention of the spindle assembly checkpoint and
Gisselsson D, Hakanson U, Stoller P, Marti D, Jin Y, Rosengren AH, Stewenius Y, Kahl F, Panagopoulos I. PLoS One. 2008 Apr 2;3(4):e1871.
The reversibility of mitotic exit in vertebrate cells. - The reversibility of mitotic exit in vertebrate cells.
Potapova TA, Daum JR, Pittman BD, Hudson JR, Jones TN, Satinover DL, Stukenberg PT, Gorbsky GJ. Nature. 2006 Apr 13;440(7086):954-8.