Anti-Cyclin D1 antibody [EPR2241]

• Supplier: Abcam
• Catalog: ab134175
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2241
• Applications: WB IP ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cyclin D1 (C terminal)
• Blocking Peptide: Cyclin D1 peptide
• Description: Rabbit Monoclonal
• Gene: CCND1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Cyclin D1 antibody [EPR2241] (ab134175) at 1/10000 dilution (purified)Lane 1 : Mouse kidneyLane 2 : Mouse spleenLane 3 : Rat heartLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of paraffin embedded human endometrial adenocarcinoma with purified ab134175 at a dilution of 1/100. A HRP goat anti-rabbit (ab97051) was used as the secondary antibody at a dilution of 1/500 and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunofluorescent staining of Ramos cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab134175 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counter stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

All lanes : Anti-Cyclin D1 antibody [EPR2241] (ab134175) at 1/100000 dilution (purified)Lane 1 : MCF-7 cell lysateLane 2 : LnCap cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-Cyclin D1 antibody [EPR2241] (ab134175) at 1/20000 dilution (purified)Lane 1 : HeLa cell lysateLane 2 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

developed using the ECL techniquePerformed under reducing conditions.

Unpurified ab134175 staining Cyclin D1 in MCF7 cells treated with KN-93 (ab120980). The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab134175 at 10μg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Goat anti-Rabbit Alexa 488 secondary (ab150081) at 2 μg/ml (shown in green) and Goat anti-Mouse Alexa 594 secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Immunohistochemical analysis of paraffin-embedded Human mantle cell lymphoma tissue labelling Cyclin D1 with unpurified ab134175 at 1/100 dilution.

ab134175 (purified) at 1/30 immunoprecipitating cyclin D1 in A431 cells (Lane 1). For western blotting, a HRP-conjugated goat anti-rabbit IgG, was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Anti-Cyclin D1 antibody [EPR2241] (ab134175) at 1/10000 dilution (unpurified) + MCF7 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

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