Anti-Cyclin E2 antibody [E142]
| Name | Anti-Cyclin E2 antibody [E142] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32103 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | E142 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to residues in C terminus of human Cyclin E2 |
| Description | Rabbit Monoclonal |
| Gene | CCNE2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Cyclin E2 antibody [E142] (ab32103) at 1/500 dilution + HeLa cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/4084_Wb%2520cyclin%2520e2.jpg)
Anti-Cyclin E2 antibody [E142] (ab32103) at 1/500 dilution + HeLa cell lysate
Immunofluorescent analysis of Cyclin E2 expression in HeLa cell culture using 1/100 ab32103.
Immunohistochemical analysis of Cyclin E2 expression in paraffin embedded human breast carcinoma using 1/50 ab32103.
Overlay histogram showing HeLa cells stained with ab32103 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32103, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Mammalian E-type cyclins control chromosome pairing, telomere stability and CDK2 - Mammalian E-type cyclins control chromosome pairing, telomere stability and CDK2
Martinerie L, Manterola M, Chung SS, Panigrahi SK, Weisbach M, Vasileva A, Geng Y, Sicinski P, Wolgemuth DJ. PLoS Genet. 2014 Feb 27;10(2):e1004165.
Osteocalcin promotes beta-cell proliferation during development and adulthood - Osteocalcin promotes beta-cell proliferation during development and adulthood
Wei J, Hanna T, Suda N, Karsenty G, Ducy P. Diabetes. 2014 Mar;63(3):1021-31.
Ubiquitin-recognition protein Ufd1 couples the endoplasmic reticulum (ER) stress - Ubiquitin-recognition protein Ufd1 couples the endoplasmic reticulum (ER) stress
Chen M, Gutierrez GJ, Ronai ZA. Proc Natl Acad Sci U S A. 2011 May 31;108(22):9119-24. doi: