All lanes : Anti-CYP11A1 antibody (ab86357) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 3 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP))developed using the ECL techniquePerformed under reducing conditions.