Anti-CYP46 antibody
| Name | Anti-CYP46 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab82814 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Mouse, Rat, Goat, Horse, Guinea Pig, Bovine, Cat, Dog |
| Antigen | synthetic peptide corresponding to a region within internal amino acids 396-445 (YVMGRMDTYF EDPLTFNPDR FGPGAPKPRF TYFPFSLGHR SCIGQQFAQM) of human CYP46 (NP_006659) |
| Description | Rabbit Polyclonal |
| Gene | CYP46A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab82814 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab82814 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-CYP46 antibody (ab82814) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution