Anti-Cysteine Dioxygenase Type 1 antibody

• Supplier: Abcam
• Catalog: ab53436
• Prices: $359.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IP IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant full length protein (Rat)
• Description: Rabbit Polyclonal
• Gene: CDO1
• Conjugate: Unconjugated

Product images

ICC/IF image of ab53436 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53436, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab53436 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53436, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma - Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma

Prabhu A, Sarcar B, Kahali S, Yuan Z, Johnson JJ, Adam KP, Kensicki E, Chinnaiyan P. Cancer Res. 2014 Feb 1;74(3):787-96.

TonEBP stimulates multiple cellular pathways for adaptation to hypertonic stress: - TonEBP stimulates multiple cellular pathways for adaptation to hypertonic stress:

Lee SD, Choi SY, Lim SW, Lamitina ST, Ho SN, Go WY, Kwon HM. Am J Physiol Renal Physiol. 2011 Mar;300(3):F707-15. doi: