Anti-Cytochrome b5 antibody
| Name | Anti-Cytochrome b5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab69801 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide derived from an internal region of Human Cytochrome b5 |
| Description | Rabbit Polyclonal |
| Gene | CYB5A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Cytochrome b5 antibody (ab69801) at 1/500 dilutionLane 1 : extracts from A549 cellsLane 2 : extracts from A549 cells with immunizing peptide at 10 µgLysates/proteins at 15 µg per lane.
Left: NIH/3T3 cells stained with ab69801 at 1/500 dilution; Right: the antibody was pre-incubated with the immunizing peptide.
IHC image of ab69801 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69801, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
AMP activated protein kinase is indispensable for myocardial adaptation to - AMP activated protein kinase is indispensable for myocardial adaptation to
Chen K, Kobayashi S, Xu X, Viollet B, Liang Q. PLoS One. 2013;8(3):e59682.
Protein expression profiling of nuclear membrane protein reveals potential - Protein expression profiling of nuclear membrane protein reveals potential
Khan R, Zahid S, Wan YJ, Forster J, Karim AB, Nawabi AM, Azhar A, Rahman MA, Ahmed N. Clin Proteomics. 2013 Jun 1;10(1):6.
A missense mutation in the human cytochrome b5 gene causes 46,XY disorder of sex - A missense mutation in the human cytochrome b5 gene causes 46,XY disorder of sex
Idkowiak J, Randell T, Dhir V, Patel P, Shackleton CH, Taylor NF, Krone N, Arlt W. J Clin Endocrinol Metab. 2012 Mar;97(3):E465-75.
Cytochrome b5 and cytokeratin 17 are biomarkers in bronchoalveolar fluid - Cytochrome b5 and cytokeratin 17 are biomarkers in bronchoalveolar fluid
Menoret A, Kumar S, Vella AT. PLoS One. 2012;7(7):e40184.
Cytochrome b(5) shifts oxidation of the anticancer drug ellipticine by - Cytochrome b(5) shifts oxidation of the anticancer drug ellipticine by
Kotrbova V, Mrazova B, Moserova M, Martinek V, Hodek P, Hudecek J, Frei E, Stiborova M. Biochem Pharmacol. 2011 Sep 15;82(6):669-80.