Anti-Cytochrome P450 2D6 antibody
| Name | Anti-Cytochrome P450 2D6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab62204 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Human, Mouse, Rat, Sheep, Rabbit, Horse, Guinea Pig, Bovine, Cat, Pig, Chimpanzee, Zebrafish |
| Antigen | A region within synthetic peptide: ILGFGPRSQG VFLARYGPAW REQRRFSVST LRNLGLGKKS LEQWVTEEAA, corresponding to N terminal amino acids 109-158 of Human Cytochrome P450 2D6 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | CYP2D6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Cytochrome P450 2D6 antibody (ab62204) at 2.5 µg/ml + Fetal liver cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG 1/ 50,000 - 1/100,000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cytochrome P450 2D6 with ab62204 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
ICC/IF image of ab62204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.