Anti-Cytochrome P450 2E1 antibody
| Name | Anti-Cytochrome P450 2E1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28146 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Rabbit, Horse, Guinea Pig, Dog, Human, Monkey |
| Antigen | Full length native protein (purified) (Rat) |
| Description | Rabbit Polyclonal |
| Gene | CYP2E1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab28146 staining cells of rat liver sections by IHC-Fr. Cells were fixed in acetone and blocked with 1% BSA for 5 minutes at 25°C prior to incubating with ab28146 diluted 1/300 for 30 minutes at 25°C. An Alexa Fluor® 594 conjugated goat anti-rabbit antibody was used as the secondary.Double IF labeling with CYP2E1 (Red) and dipeptidyl peptidase IV (Green)See Abreview
ICC/IF image of ab28146 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28146, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/1000 dilutionLane 1 : Molecular weight markerLane 2 : Cell lysates prepared from human liver microsomesLane 3 : Cell lysates prepared from rat liver microsomesLane 4 : Cell lysates prepared from mouse liver microsomesLane 5 : Cell lysates prepared from rabbit liver microsomes
All lanes : Anti-Cytochrome P450 2E1 antibody (ab28146) at 1/2500 dilutionLane 1 : Tissue lysate prepared from normal murine liverLane 2 : Tissue lysate prepared from normal murine liverLane 3 : Tissue lysate prepared from normal murine liverLane 4 : Tissue lysate prepared from normal murine liverLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG-HRP at 1/5000 dilutiondeveloped using the ECL technique
Product References
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