Anti-Cytochrome P450 3A4 antibody
| Name | Anti-Cytochrome P450 3A4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3572 |
| Prices | $382.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Other Immunogen Type (His-tag) corresponding to Human Cytochrome P450 3A4 |
| Description | Rabbit Polyclonal |
| Gene | CYP3A4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab3572 (1µg/ml) staining cytochrome P450 3A4 in human Liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasm of the plates of hepatic cells .Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Anti-Cytochrome P450 3A4 antibody (ab3572) at 1/500 dilution + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
The effect of microRNAs in the regulation of human CYP3A4: a systematic study - The effect of microRNAs in the regulation of human CYP3A4: a systematic study
Wei Z, Jiang S, Zhang Y, Wang X, Peng X, Meng C, Liu Y, Wang H, Guo L, Qin S, He L, Shao F, Zhang L, Xing Q. Sci Rep. 2014 Mar 5;4:4283.
No correlation of hsa-miR-148a with expression of PXR or CYP3A4 in human livers - No correlation of hsa-miR-148a with expression of PXR or CYP3A4 in human livers
Wei Z, Chen M, Zhang Y, Wang X, Jiang S, Wang Y, Wu X, Qin S, He L, Zhang L, Xing Q. PLoS One. 2013;8(3):e59141.
Induction of CYP3A4 and MDR1 gene expression by baicalin, baicalein, chlorogenic - Induction of CYP3A4 and MDR1 gene expression by baicalin, baicalein, chlorogenic
Li Y, Wang Q, Yao X, Li Y. Eur J Pharmacol. 2010 Aug 25;640(1-3):46-54.
Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing - Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing
Irusta G, Murphy MJ, Perez WD, Hennebold JD. Mol Hum Reprod. 2007 Aug;13(8):541-8. Epub 2007 Jun 12.