All lanes : Anti-Cytochrome P450 3A4 antibody (ab135813) at 1/50 dilutionLane 1 : NCI-H460 cell line lysates Lane 2 : NCI-H460 cell line lysates with blocking peptideLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human liver tissue labelling Cytochrome P450 3A4 with ab135813 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of CEM cells labelling Cytochrome P450 3A4 with ab135813 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.