Anti-Cytochrome P450 3A5 antibody [EPR4396]

• Supplier: Abcam
• Catalog: ab108624
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4396
• Applications: WB IP IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cytochrome P450 3A5 aa 250-350
• Description: Rabbit Monoclonal
• Gene: CYP3A5
• Conjugate: Unconjugated

Product images

Anti-Cytochrome P450 3A5 antibody [EPR4396] (ab108624) at 1/400 dilution (unpurified) + Human fetal liver tissue lysate at 20 µgSecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Anti-Cytochrome P450 3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (purified) + Human fetal liver tissue lysate at 20 µgSecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-Cytochrome P450 3A5 antibody [EPR4396] (ab108624) at 1/1000 dilution (unpurified)Lane 1 : Human fetal liver tissue lysateLane 2 : Human fetal colon tissue lysateLysates/proteins at 10 µg per lane.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Cytochrome P450 3A5 with unpurified ab108624 at 1/80. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Cytochrome P450 3A5 with purified ab108624 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Positive staining is shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human kidney tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Positive staining is shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human breast tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Negative staining is shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Negative staining is shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labelling Cytochrome P450 3A5 with unpurified ab108624. Negative staining is shown.

ab108624 (unpurified) at 1/20 immunoprecipitating Cytochrome P450 3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

ab108624 (purified) at 1/50 immunoprecipitating Cytochrome P450 3A5 in human fetal liver tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.