Anti-Cytoglobin antibody
| Name | Anti-Cytoglobin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57713 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ELISA IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-191 of Human Cytoglobin |
| Description | Mouse Monoclonal |
| Gene | CYGB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab57713 at 3µg/ml, staining cytoglobin in human heart tissue by IHC-P.
All lanes : Anti-Cytoglobin antibody (ab57713) at 1/500 dilutionLane 1 : Transfected 293T lysate at 25 µgLane 2 : Non-transfected 293T lysate at 25 µg
![Overlay histogram showing HeLa cells stained with ab57713 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57713, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5041_Cytoglobin-Primary-antibodies-ab57713-3.jpg)
Overlay histogram showing HeLa cells stained with ab57713 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57713, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.