Anti-Cytokeratin 14 antibody [EP1612Y]
| Name | Anti-Cytokeratin 14 antibody [EP1612Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51054 |
| Prices | $400.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1612Y |
| Applications | WB IP FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to residues near the C terminus of human Cytokeratin 14 |
| Description | Rabbit Monoclonal |
| Gene | KRT14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab51504 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51504, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-Cytokeratin 14 antibody [EP1612Y] (ab51054) at 1/20000 dilution + A431 cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5193_ab51054_1.bmp)
Anti-Cytokeratin 14 antibody [EP1612Y] (ab51054) at 1/20000 dilution + A431 cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution
Overlay histogram showing A431 cells stained with ab51054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51054,1/100 dilution ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
ab51054 staining Cytokeratin 14 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer, pH 6.0. Samples were then permeabilized using 0.1% saponin/PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab51054 at a 1/200 dilution for 16 hours at 4°C. The secondary used was a Texas Red conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.See Abreview
Product References
Proteomic profile of keratins in cancer of the gingivo buccal complex: - Proteomic profile of keratins in cancer of the gingivo buccal complex:
Fulzele A, Malgundkar SA, Govekar RB, Patil A, Kane SV, Chaturvedi P, D'Cruz AK, Zingde SM. J Proteomics. 2013 Oct 8;91:242-58.
Reconstruction of a human hemicornea through natural scaffolds compatible with - Reconstruction of a human hemicornea through natural scaffolds compatible with
Barbaro V, Ferrari S, Fasolo A, Ponzin D, Di Iorio E. Mol Vis. 2009 Oct 17;15:2084-93.
Correction of laminin-5 deficiency in human epidermal stem cells by - Correction of laminin-5 deficiency in human epidermal stem cells by
Di Nunzio F, Maruggi G, Ferrari S, Di Iorio E, Poletti V, Garcia M, Del Rio M, De Luca M, Larcher F, Pellegrini G, Mavilio F. Mol Ther. 2008 Dec;16(12):1977-85.