Anti-Cytokeratin 14 antibody [LL002]
| Name | Anti-Cytokeratin 14 antibody [LL002] |
|---|---|
| Supplier | Abcam |
| Catalog | ab49806 |
| Prices | $385.00 |
| Sizes | 200 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG3 |
| Clone | LL002 |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide: GKV VSTHEQVLRT KN , corresponding to amino acids 458-472 of Human Cytokeratin 14 conjugated to thyroglobulin |
| Description | Mouse Monoclonal |
| Gene | KRT14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab49806 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49806, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-Cytokeratin 14 antibody [LL002] (ab49806) at 1/500 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5228_Cytokeratin-14-Primary-antibodies-ab49806-2.jpg)
Anti-Cytokeratin 14 antibody [LL002] (ab49806) at 1/500 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Human normal skin. Staining is observed in the cytoplasm. Left panel: with primary antibody diluted 1:2000. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.