Anti-Cytokeratin 14 antibody [RCK107]
| Name | Anti-Cytokeratin 14 antibody [RCK107] |
|---|---|
| Supplier | Abcam |
| Catalog | ab9220 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | RCK107 |
| Applications | DB ICC/IF IHC-F WB IHC-P FC |
| Species Reactivities | Rat, Dog, Human, Pig |
| Antigen | Cytoskeletal preparation of TR146 epithelial cells |
| Description | Mouse Monoclonal |
| Gene | KRT14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab9220 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9220, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-Cytokeratin 14 antibody [RCK107] (ab9220) at 4 µg/ml + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 20 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 58 kDa (why is the actual band size different from the predicted?)Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.Exposure time : 20 minutes](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/5236_Cytokeratin-14-Primary-antibodies-ab9220-6.jpg)
Anti-Cytokeratin 14 antibody [RCK107] (ab9220) at 4 µg/ml + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 20 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 58 kDa (why is the actual band size different from the predicted?)Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.Exposure time : 20 minutes
Product References
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