Anti-Cytokeratin 15 antibody [EPR1614Y]

• Supplier: Abcam
• Catalog: ab52816
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1614Y
• Applications: ICC/IF ICC/IF WB FC IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to residues from the C-terminus of human Cytokeratin 15
• Description: Rabbit Monoclonal
• Gene: KRT15
• Conjugate: Unconjugated

Product images

Anti-Cytokeratin 15 antibody [EPR1614Y] (ab52816) at 1/10000 dilution + fetal thymus lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution

ab52816 at 1/100 dilution staining Cytokeratin 15 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.

ab52816 at 1/50 dilution staining Cytokeratin 15 in HeLa cells by Immunocytochemistry.

Overlay histogram showing A431 cells stained with ab52816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52816,1/100 dilution ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

Fluorescent immunohistochemical analysis of paraffin-embedded human normal skin tissue using ab52816. Green-CK15 red-PI

Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using ab52816. Green-CK15 red-PI.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

The expression profiles of acidic epithelial keratins in ameloblastoma. - The expression profiles of acidic epithelial keratins in ameloblastoma.

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Cytokeratin 15 marks basal epithelia in developing ureters and is upregulated in - Cytokeratin 15 marks basal epithelia in developing ureters and is upregulated in

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Tenascin-C and tenascin-W in whisker follicle stem cell niches: possible roles in - Tenascin-C and tenascin-W in whisker follicle stem cell niches: possible roles in

Tucker RP, Ferralli J, Schittny JC, Chiquet-Ehrismann R. J Cell Sci. 2013 Nov 15;126(Pt 22):5111-5.

Transplantation of human embryonic stem cells onto a partially wounded human - Transplantation of human embryonic stem cells onto a partially wounded human

Hanson C, Hardarson T, Ellerstrom C, Nordberg M, Caisander G, Rao M, Hyllner J, Stenevi U. Acta Ophthalmol. 2013 Mar;91(2):127-30.

Reduction of NOTCH1 expression pertains to maturation abnormalities of - Reduction of NOTCH1 expression pertains to maturation abnormalities of

Sakamoto K, Fujii T, Kawachi H, Miki Y, Omura K, Morita K, Kayamori K, Katsube K, Yamaguchi A. Lab Invest. 2012 May;92(5):688-702.

Generating CK19-positive cells with hair-like structures from Wharton's jelly - Generating CK19-positive cells with hair-like structures from Wharton's jelly

Aljitawi OS, Xiao Y, Zhang D, Stehno-Bittel L, Garimella R, Hopkins RA, Detamore MS. Stem Cells Dev. 2013 Jan 1;22(1):18-26.

A case of primary combined neuroendocrine carcinoma with squamous cell carcinoma - A case of primary combined neuroendocrine carcinoma with squamous cell carcinoma

Mochizuki Y, Omura K, Sakamoto K, Nakanishi S, Satoh K, Marukawa E, Yamaguchi A. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Apr;109(4):e34-9. doi: