All lanes : Anti-Cytokeratin 16 antibody (ab53117) at 1/300 dilutionLane 1 : HepG2 cell extractLane 2 : HepG2 cell extract with immunising peptide
ab53117 at 1/50 dilution staining Cytokeratin 16 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
ICC/IF image of ab53117 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53117, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab53117 staining Cytokeratin 6 in transplanted Human skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% Goat serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour at 22°C. An HRP-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. See Abreview