Anti-Cytokeratin 16 antibody [EP1615Y]

• Supplier: Abcam
• Catalog: ab76416
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1615Y
• Applications: IHC-P WB IP ICC/IF FC
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues near the N terminus of human Cytokeratin 16
• Description: Rabbit Monoclonal
• Gene: KRT16
• Conjugate: Unconjugated

Product images

Anti-Cytokeratin 16 antibody [EP1615Y] (ab76416) at 1/10000 dilution + HaCat cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of Cytokeratin 16 in paraffin embedded human squamous cervical carcinoma tissue using ab76416 at a 1/100 dilution.

Overlay histogram showing HepG2 cells stained with ab76416 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76416, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

Product References

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